Journal: Journal of Proteome Research

Article Title: LC–MS Proteomics Analysis of the Insulin/IGF-1-Deficient Caenorhabditis elegans daf-2(e1370) Mutant Reveals Extensive Restructuring of Intermediary Metabolism

doi: 10.1021/pr401081b

Figure Lengend Snippet: Mitochondrial intermediary metabolism. (Left) Heat map (base-2 logarithmic scale) and (right) schematic overview of enzymes in mitochondrial intermediary metabolism. Numbers next to each heat map row denote fold change (linear) in abundance for statistically significant proteins. Proteins with significantly changed abundance levels are denoted *, p < 0.05; **, p < 0.005; and ***, p < 0.0005. Inset bar graphs represent relative enzymatic activities of aconitase, fumarase, and isocitrate lyase. (1) Pyruvate dehydrogenase complex, (2) citrate synthase, (3) aconitase, (4) isocitrate dehydrogenase complex, (5) α-ketoglutarate dehydrogenase complex (E1, oxoglutarate dehydrogenase; E2, dihydrolipoyl succinyl transferase; and E3, dihydrolipoyl dehydrogenase), (6) succinate thiokinase, (7) succinate dehydrogenase, (8) fumarase, (9) malate dehydrogenase, (10) pyruvate carboxylase, (11) isocitrate lyase, (12) malate synthase, (13) mitochondrial tri/dicarboxylate carrier protein, (14) GDH-1, glutamate dehydrogenase; W07E11.1, glutamate synthase, (15) malate-α-ketoglutarate antiporter (malate shuttle), and (16) aspartate aminotransferase. Succ, succinate; succ-CoA, succinyl-CoA; ACO, aconitase; and FUM, fumarase.

Article Snippet: The carbon atoms of propionate are recycled into the citric acid cycle component succinyl-CoA via an evolutionary conserved multienzyme pathway that was shown to be fully functional in C. elegans (Figure )., We detected significantly increased abundance of the propionate catabolic enzymes methylmalonyl-CoA racemase (MCE-1), methylmalonyl-CoA mutase (MMCM-1), and the alpha (PCCA-1) and beta (PCCB-1) subunits of propionyl-CoA decarboxylase in daf-2 adult animals (Figure ).

Techniques:

Journal: Journal of Proteome Research

Article Title: LC–MS Proteomics Analysis of the Insulin/IGF-1-Deficient Caenorhabditis elegans daf-2(e1370) Mutant Reveals Extensive Restructuring of Intermediary Metabolism

doi: 10.1021/pr401081b

Figure Lengend Snippet: Fatty acid metabolism. (A) Synthesis of fatty acids. (Right) Heat map and (left) schematic overview of enzymes implicated in fatty acid synthesis. (1) Citrate synthase, (2) mitochondrial tricarboxylate/dicarboxylate carrier protein (citrate transport protein), (3) ATP-citrate lyase, and (4) FASN-1, fatty acid synthase; ELO-1, long-chain fatty acid elongase; DHS-25, mitochondrial beta-ketoacyl-ACP reductase; FAT-6 and FAT-2 encode fatty acyl desaturases. 3-HADH, 3-hydroxyacyl-CoA dehydrogenase. (B) Oxidation of fatty acids. (Right) Heat map and (left) schematic overview of detected fatty acid β-oxidation enzymes. Numbers next to each heat map row denote fold change (linear) in abundance for statistically significant proteins. Proteins with significantly changed abundance levels are denoted *, p < 0.05; **, p < 0.005; and ***, p < 0.0005. Inset bar graph represent relative enzymatic activity of acyl-CoA dehydrogenase. (1) Acyl-CoA synthetase, (2) carnitine O-acyltransferase, (3) acyl-CoA dehydrogenase, (4) trans-2-enoyl-CoA hydratase, (5) 3-hydroxyacyl-CoA dehydrogenase, (6) thiolase, (7) acetyl-CoA acetyltransferase (thiolase), (8) 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) lyase, and (9) succinyl-CoA-acetoacetate CoA transferase.

Article Snippet: The carbon atoms of propionate are recycled into the citric acid cycle component succinyl-CoA via an evolutionary conserved multienzyme pathway that was shown to be fully functional in C. elegans (Figure )., We detected significantly increased abundance of the propionate catabolic enzymes methylmalonyl-CoA racemase (MCE-1), methylmalonyl-CoA mutase (MMCM-1), and the alpha (PCCA-1) and beta (PCCB-1) subunits of propionyl-CoA decarboxylase in daf-2 adult animals (Figure ).

Techniques: Activity Assay